(A) CYC1s in vitro polyadenylation reactions carried out for 30 min in wt or rrp6Δ extracts before (total) or after (dep) no TAP-, Pab1p-TAP- or Nab2p-TAP-depletion as indicated. Pab1p-TAP and Nab2p-TAP proteins were depleted using rabbit IgG antibody. Image labeling as in Figure 1B.
(B) CYC1s in vitro polyadenylation reactions carried out for 10 or 30 min in wt extracts supplemented with buffer (lanes 2 and 3), 0.5-fold (lanes 4 and 5) or 2-fold (lanes 6 and 7) excess recombinant Pab1; or 2-fold (lanes 8 and 9) or 8-fold (lanes 10 and 11) excess recombinant Nab2 as indicated.
See also Figure S4.