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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Nature. 2012 Jul 26;487(7408):443–448. doi: 10.1038/nature11314

Figure 1. MCT1 expressed primarily within oligodendroglia in the CNS.

Figure 1

(a) Histogram and rtPCR for MCT1 mRNA from FAC sorted cells with (b) or without (a) tdTomato expression. (b–e) Corpus callosum of tdTomato MCT1 reporter mice crossed with BAC-MOBP eGFP (b,c) or BAC-GLT1 eGFP (d,e) mice. (f–i) Spinal cord of tdTomato MCT1 reporter mice crossed with BAC-MOBP eGFP (f,g) or BAC-GLT1 eGFP (h,i) mice (Scale bars = 50 and 14 μm for low and high power, respectively). (j–o) TdTomato MCT1 reporter mouse brain immunostained with cell-specific markers (scale bars = 12.5 μm, except 50 μm for lectin). (p) Percentage of tdTomato cells co-labelled with CNS cell markers in the spinal cord (blue), cortex (red), and corpus callosum (green). (q,r) Real-time rtPCR of MCT1 mRNA isolated from oligodendroglia and astroglia by FACS and BacTRAP techniques (q; means of 4 replicates) and human oligodendroglioma (M03.13) and astrocytoma (U87) cell lines (r). (s) Lactate transport and blockade by selective MCT1 inhibitor in oligodendroglioma and astrocytoma.