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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Nature. 2012 Jul 26;487(7408):443–448. doi: 10.1038/nature11314

Figure 2. MCT1 required for neuronal survival in vitro.

Figure 2

(a–d) Photomicrograph (a) and quantification of motoneurons in spinal cord slice cultures treated with media only (Ctrl), MCT1 sense oligonucleotides (Sense), or MCT1 antisense oligonucleotides (ASO) for 3 weeks (b; n ≥ 55 sections per group), following 3 weeks treatment with MCT1i (c; n ≥ 27 sections per group), or 2 hrs of glucose deprivation (GD) ± MCT1i (d; n ≥ 30 sections per group). (e,f) Propidium iodide (PI) uptake in slice cultures treated with 2 hrs GD + MCT1i (e; n ≥ 10 sections per group) or 2 hrs GD, ± MCT1i, ± 20 mM lactate (f; n = 15 for all groups). (g,h) PI uptake in slices treated with GD + MCT1i labelled with neuronal (g; co-localized cells marked with arrowheads) or oligodendroglia (h) marker. (i) Percentage of PI-labelled cells co-localizing cell-specific markers (n 10 sections per group, Error bars = ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001).