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. Author manuscript; available in PMC: 2013 Jul 27.
Published in final edited form as: Chem Biol. 2012 Jun 28;19(7):913–925. doi: 10.1016/j.chembiol.2012.05.018

Figure 4. BODIPY-cholesterol accumulates within an endocytic compartment that is distinct from lipid droplets.

Figure 4

(A&B) Confocal images (63X) showing transferrin-Alexa Fluor 555 (transferrin) or AM1-43 labeling of the endocytic compartment in live 6-dpf larval ENTs. All larvae were fed a high-fat diet containing BODIPY-cholesterol. Each image is representative of images taken from ≥3 experiments.

(A) AM1-43-labeled endocytic compartments (red) colocalize with BODIPY-cholesterol labeled enrichments (green) during feeding. Adjusted PCC in ROI = 0.46; thresholded MA, AM143 = 0.95; thresholded MB, AM1-43 = 0.99.

(B) Transferrin-labeled endocytic compartments (magenta) co-localize with BODIPY-cholesterol labeled enrichments (green) during feeding. Adjusted PCC in ROI volume = 0.28; thresholded MA, Tf555 = 0.89; thresholded MB, Tf555 = 0.84.