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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Genet. 2012 Jul 15;44(8):861–871. doi: 10.1038/ng.2350

Figure 3. Blk does not suppress normal HSCs.

Figure 3

(a, b and c) FACS analysis of HSCs, CMP, GMP and MEP cells in bone marrow of WT (n=3) or Blk−/− (n=4) mice. (b) The percentages of LSK, LT-HSCs, and ST-HSCs in bone marrow of WT or Blk−/− mice. (c) The percentages of CMP, GMP, and MEP cells in bone marrow of WT (n=3) or Blk−/− (n=4) mice. (d) The percentages of myeloid (Gr-1+Mac-1+) and lymphoid (B220+IgM+) cells in bone marrow of WT or Blk−/− mice. (e) Cell cycle analysis of LSK cells in bone marrow of WT or Blk−/− mice. (f) Apoptosis of bone marrow cells and LSK cells from WT (n=3) or Blk−/− (n=4) mice. (g) FACS analysis of different donor cell lineages in recipient mice at 8, 12 and 16 weeks after transplantation. * P<0.05 (h) Colony forming assay of WT and Blk−/− bone marrow cells. (i) Three doses (1×105, 5×105, 2.5×104) of WT or Blk−/− BM cells were injected into lethally irradiated recipients, and survival of the mice were compared. (j) FACS analysis of cell lineages in peripheral blood of recipients of Blk and vector transduced bone marrow cells at 8, 12, 16 weeks after transplantation. (k) The percentages of GFP+ LSK cells in bone marrow of recipients of vector and Blk transduced bone marrow cells at 16 weeks after transplantation. (l) Cell cycle analysis of LSK cells from bone marrow of recipients of GFP or Blk/GFP transduced bone marrow cells. (P=0.86 for G0-G1; P=0.2 for S+G2M).