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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Genet. 2012 Jul 15;44(8):861–871. doi: 10.1038/ng.2350

Figure 5. c-Myc and EBF1 regulate Pax5 expression.

Figure 5

(a) ChIP assay showed c-Myc directly bound to the Pax5 promoter. Results were shown as mean ± s.e.m. (b) A Pax5 promoter luciferase reporter construct was cotransfected with empty vector or c-Myc plasmid into NIH3T3 cells. Cell extracts were analyzed for luciferase activity. Results were shown as mean ± s.e.m.. (c) Luciferase assay showed mutant c-Myc binding site in the Pax5 promoter restored the luciferase activity. Results were shown as mean ± s.e.m. (d and e) Real time RT-PCR analysis monitoring Pax5 and Blk expression in c-Myc-expressing or Pax5-expressing LSK cells. Results were shown as mean ± s.e.m. (f) ChIP assay showed EBF1 directly bind to the Pax5 promoter. Results were shown as mean ± s.e.m. (g) A Pax5 promoter luciferase reporter construct was cotransfected with empty vector, EBF1, or IRF8 plasmids into NIH3T3 cells. Cell extracts were analyzed for luciferase activity. Results were shown as mean ± s.e.m. (h) Luciferase assay showed mutant EBF1 binding site in the Pax5 promoter rescued the luciferase activity. Results were shown as mean ± s.e.m. (i) Real time RT-PCR analysis monitoring EBF1 expression by BCR-ABL in LSCs as compared to normal HSCs. Results are given as mean ± s.e.m. (j) Real time RT-PCR analysis monitoring EBF1 expression in c-Myc-expressing LSK cells. Results are given as mean ± s.e.m.