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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Genet. 2012 Jul 15;44(8):861–871. doi: 10.1038/ng.2350

Figure 6. p27 is a downstream partner of Blk in LSCs.

Figure 6

(a) Western blot analysis of the expression of p27, p21, Cdk2 and Skp2 in 293T cells after transfection with BCR-ABL, Blk or BCR-ABL-Blk. (b) Western blot analysis monitoring p27 expression. BCR-ABL-transduced bone marrow cells from WT or Blk−/− mice were grown in Whitlock-Witte culture for 7 days, and protein lysates were isolated for comparing p27 expression regulated by BCR-ABL in the presence and absence of BCR-ABL by Western blotting. (c) Microarray analysis showing Cdkn1b expression in vector-, BCR-ABL- and BCR-ABL-Blk-transduced LSCs. Mean values (± s.e.m) are shown. (d) The total numbers of total LSCs, LT-LSCs, and ST-LSCs in bone marrow of recipients of BCR-ABL-transduced Cdkn1b−/− (n=4) and BCR-ABL-transduced WT (n=3) donor bone marrow cells at day 14 after BMT. Mean values (± s.e.m.) are shown. (*P<0.05) (e) Kaplan-Meier survival curves for recipients of BCR-ABL-transduced bone marrow cells from WT or Cdkn1b−/− donor mice (P=0.02; n=8 for each group). (f) FACS analysis showing the percentages of GFP+Gr-1+ cells in peripheral blood of recipients of BCR-ABL-transduced bone marrow cells from WT or Cdkn1b−/− donor mice at day 12 after BMT. (P<0.02). (g) The total numbers of GFP+Gr-1+ cells in peripheral blood of recipients of BCR-ABL-transduced bone marrow cells from WT or Cdkn1b−/− donor mice at days 12 and 16 after BMT. Mean values (± s.e.m.) are shown.