a, Number of GFP-positive metastatic nodules in the lungs of mice bearing subcutaneous tumours established with Vec- or Wnt2-expressing NB508 cells. b, Number of GFP-positive CTCs captured from the blood of mice described above. c, Number of GFP-positive lung metastatic nodules in mice, following tail vein injection with Vec- or Wnt2-NB508 cells tagged with both GFP and luciferase. d, Representative images of tumour spheres formed by Vec- or Wnt2-NB508 cells plated at 1,000 cells/well (scale bar = 250 µm). Quantitation of both tumour sphere number and diameter is shown plated at 100 cells/well (n=3). e, Immunoblotting analysis of Vec- and Wnt2-NB508 cells at time intervals following plating under non-adherent conditions. f, Suppression of tumour spheres formed by Wnt2-NB508 cells following infection with lentivirus encoding shRNA targeting Fn1 compared with non-target (NT) shRNA (n=3). Effectiveness of knockdown is shown by western blot above. (mean ± s.d.; * p < 0.01,** p < 0.001).