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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Toxicology. 2012 Jun 12;300(1-2):75–82. doi: 10.1016/j.tox.2012.06.001

Fig. 5.

Fig. 5

Caffeine induces MeHg tolerance and elevated CYP expression. (A) Developmental toxicity of caffeine is determined by eclosion assays with Canton S larva on food containing indicated concentrations of caffeine. No significant decrease in eclosion rate is seen up to 2 mM caffeine. Significant decreases in eclosion rate are seen for 5, 10 and 20 mM caffeine. (B) Eclosion assays of Canton S larvae reared on food with indicated concentrations of caffeine and MeHg (n = 150 flies/data point for all assays in (A) and (B). Eclosion is expressed as mean and s.d. *: p = <0.05; **: p = <0.005 determined by Student’s t-test at each MeHg concentration relative to no caffeine control). (C) Expression level CYP6g1 and CYP12d1-p levels are determined by qRT-PCR with RNA from extracts of Canton S whole larvae reared on food with the indicated concentration of caffeine (pooled samples of 25 larvae for each caffeine concentration). Expression levels for both genes are normalized to CYP6g1 expression with no caffeine.