Skip to main content
. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: J Immunol Methods. 2009 Nov 15;352(1-2):38–44. doi: 10.1016/j.jim.2009.11.005

Figure 1.

Figure 1

Schematic diagram of the sandwich ELISA. The sandwich ELISA used in this study involves (1) immobilization of capture antibody (CAb) to the 96 well plate, (2) capture of rm-Wnt5a via the CAb, (3) detection of rm-Wnt5a by detection antibody (DAb), (4) recognition of DAb by an enzyme-linked antibody (EAb), (5) addition of OPD and (6) optical analysis of signal (colored product) generated from the enzyme-substrate reaction.