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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: J Immunol Methods. 2009 Nov 15;352(1-2):38–44. doi: 10.1016/j.jim.2009.11.005

Figure 2.

Figure 2

(A) Rabbit anti-human Wnt5a readily binds to 96 well plates. The binding of rabbit anti-human Wnt5a to 96 well plates at different concentrations was determined using a direct binding ELISA. HRP conjugated F(ab’)2 donkey anti-rabbit IgG was used to detect the rabbit anti-human Wnt5a. The absorbance was measured after 45 min of the enzyme-substrate reaction. (B) HRP conjugated F(ab’)2 donkey anti-goat IgG does not cross react with rabbit anti-human Wnt5a immobilized on the plate. OD was measured after 15 min of the enzyme-substrate reaction. All values are mean ± SD of triplicate wells. *p≤0.05, compared with the other bars. The results represent one of three experiments.