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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: J Immunol Methods. 2009 Nov 15;352(1-2):38–44. doi: 10.1016/j.jim.2009.11.005

Figure 4.

Figure 4

Sandwich ELISA to determine whether the DAbs cross react with the CAbs. In each experiment anti-Wnt5a was adsorbed to the wells of the 96 wells plate. Subsequently the wells were treated as listed on the legends. In (A) rabbit anti-human Wnt5a served as the capture antibody and in (B) goat anti-mouse Wnt5a served as the capture antibody. (A) Goat anti-mouse Wnt5a shows limited cross reactivity with rabbit anti-human Wnt5a adsorbed to the plate; (B) Rabbit anti-human Wnt5a cross reacts with goat anti-mouse Wnt5a adsorbed to the plate. OD was measured after 15 min of enzyme-substrate reaction. All values are mean ± SD of triplicate wells. * p≤0.05, compared with the other bars. The results represent one of n≥2 experiments.