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Adaptive optics microscope loop correction steps. Figure 8(a) shows an uncorrected image of the fluorescent microsphere. Figure 8(e) shows the result of closing the loop by using a loop gain factor of 0.4. The length of the bar in Fig. 8(c) is equal to the diffraction limit of the 40X (0.75 NA) objective lens, 0.45 µm. The bead was located 100 um beneath the surface of the embryo.
