Fig. 1.

Measured scatter PSF of the cryo-imaging system. Twenty measured PSFs were aligned and averaged. A background value was subtracted. Maximum intensity projections in the YZ (a) and XY (b) planes are shown. The comet tail extends approximately 100 µm and the maximal in-plane spread is 15 µm. The PSF was acquired at a resolution of 5.4x5.4x10 µm using microspheres 0.2 μm diameter