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. 2012 Jan 1;4(1):44–52. doi: 10.4161/derm.19027

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Figure 2. Treatment with UV-B, 1,25(OH)2D3 and epigenetic drugs, alone or in combination, does not exert strong effects on protein expression of Notch-1 in non-malignant and in malignant human keratinocytes. Western analysis revealed that Notch-1 protein is expressed in cultured non-malignant (HaCaT keratinocytes) and malignant (SCL-1 cells) human keratinocytes. Results of representative experiments in HaCaT keratinocytes 48 h after UV-B irradiation are shown here. Please note that treatment with UV-B [(A) 0 J; (B) 100 J; (C) 500 J] and/or 1,25(OH)2D3 (10−8 M) does not exert strong effects on protein expression of Notch-1 after 24, 48 or 72 h. Please also note that treatment with epigenetic modulating drugs (TSA, 5-Aza) does not exert strong effects, alone or in combination with UV-B and/or 1,25(OH)2D3, on protein expression of Notch-1 in HaCaT keratinocytes. Western analysis of GAPDH protein (lower band) served as control.