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Figure 3. Chk1 phosphorylation is delayed in Claspin1332-expressing cells. (A and B) HCT116 cells were sequentially transfected with siPAN and plasmids expressing Flag-Claspin1339 or Flag-Claspin1332 vector. Cells were irradiated with 15 J/m2 UV, and cells extracts were prepared and analyzed by western blot at the indicated time points.
