Table 1. Clinical samples and laboratory results of primary and secondary BUD lesions.

Date of Sample Collection	Clinical Presentation and Diameter of Lesion	Sample Type	Transport Medium	Laboratory Results
				MICa	IS2404 PCRb	IS2404 qPCRc	16S RT qPCRd	CULe
July 20, 2010	Primary nodule, 30 mm	FNAfPunch biopsy	CLSgCLS	negND	negpos	NDhND	NDND	NDND
July 13, 2011	Secondary nodule, 30 mm	FNA(1)FNA(2)	CLSPANTAj	pos (1AFBi)neg	negpos	posneg	NDND	NDND
July 21, 2011	Ulcerated secondary nodule, 25×30 mm	Punch biopsySwab (1)Swab (2)Swab (3)	PANTAPANTAPANTAPANTA	negnegNDND	NDNDnegneg	NDNDpospos	NDNDnegneg	No growthkNo growthNDND

Table 1 shows samples collected from the primary and secondary lesions in July 2010 and 2011 and the corresponding laboratory results. “Neg” indicates a negative test result, “pos” indicates a positive test result.

^aMIC, microscopic examination of acid fast bacilli (AFB) following Ziehl-Neelsen staining conducted at CHR, DITM, and INH (samples from secondary lesion only).

^bIS2404 PCR, conventional, single-step, gel-based IS2404 polymerase-chain-reaction conducted at DITM.

^cIS2404 qPCR, real-time quantitative IS2404 polymerase-chain-reaction conducted at DITM.

^d16S RT qPCR, Mycobacterium ulcerans–specific reverse-transcription real-time quantitative polymerase-chain-reaction targeting the ribosomal 16S RNA of M. ulcerans conducted at DITM.

^eCUL, mycobacterial culture on Löwenstein-Jensen medium conducted at IML red, synlab, Asklepios Gauting, Germany.

^fFNA, fine-needle aspiration.

^gCLS, Puregene cell lysis solution, Qiagen, Germany.

^hND, not done.

ⁱAFB, acid fast bacilli.

^jPANTA, transport medium for viable mycobacteria containing Polymyxin B, Amphotericin, Nalidixic acid, Trimethoprim, and Azlocillin.

^kNo growth, culture result negative, no growth of acid fast bacilli.