Skip to main content
. 2012 Jul 31;6(7):e1745. doi: 10.1371/journal.pntd.0001745

Figure 3. Detection of the native SjAgo2 protein by Western blot. A. Detection of the native SjAgo2 protein in different SWAP immunoprecipitates.

Figure 3

Lanes 1 and 5, no antibody added; lanes 2 and 6, immunoprecipitated by normal mouse IgG; lanes 3 and 7, immunoprecipitated by mAb 11E8; lanes 4 and 8, immunoprecipitated by mAb 27A9. Left panel, immunoprecipitates were detected by Western blot with mAb 11E8. Right panel, immunoprecipitates were detected by Western blot with mAb 27A9. HRP-conjugated goat anti-mouse IgG was used as a secondary antibody. The arrows indicate the specific recognition of SjAgo2 by mAbs; the asterisks indicate the band recognized by normal murine IgG. B. SWAP was resolved by 12% SDS-PAGE and detected by Western blot with mAb 27A9. The arrows indicate the two bands recognized by mAb 27A9. M, prestained protein ladder SM0671.