Skip to main content
. 2012 Jul 31;10(7):e1001368. doi: 10.1371/journal.pbio.1001368

Figure 3. Virulence and densities in naïve mice of parasites that had previously been serially passaged 21 times through mice that were sham-vaccinated or AMA-1 vaccinated, together with the progenitor parasites (ancestral) (“evaluation experiment 3”).

Figure 3

Curves (A and C) show the kinetics of five C-lines (blue) and five V-lines (red) each assayed in up to three mice. Black curve is the mean of nine mice infected with the ancestral lineage. Points on the scatterplots (B and D) are individual mice infected with ancestral parasites (filled black diamonds), C-lines (filled blue circles), or V-lines (filled red triangles). Horizontal black lines indicate mean values. V-line parasites caused more anemia than the C-lines and ancestral parasites (A–B; F 1,27 = 6.2, p = 0.02 and F 1,22 = 8.2, p = 0.008, respectively). The V-lines also reached higher total parasite densities than the ancestral parasites (C–D; F 1,22 = 12.3, p = 0.002), but the C-lines and V-lines did not differ from each other (C–D; F 1,27 = 1.6, p = 0.2).