Figure 3. Effects of CD4⁺CD25⁺ Treg cells deficiency upon chronic immobilization stress in the elevated plus maze (n = 14–16).

After 21 days of stress, mice were individually placed in a central platform facing a closed arm and allowed to freely explore the maize for 5 min. Time spent in the open arms (A) and closed arms (B) of the maze and the frequency of entry into both arms (C) in the elevated plus maze. The time spent in the respective arms was recorded. Data were analyzed using separate one-way ANOVA followed by a post hoc Newman-Keuls test. ***p<0.001, *p<0.05 vs. the non-stressed control group. Vertical bars indicate the S.E.