Figure 5. Effects of CD4⁺CD25⁺ Treg cells deficiency on the durations of immobility in the tail suspension test (n = 14–16).

After 21 days of stress, mice were isolated in each chamber and suspended upside down by taping their tails to a flat metal bar so that they could not reach the top or the sides of the chamber. The mice were left in this position for 6 min and the total duration of immobility was measured. Data were analyzed using separate one-way ANOVA followed by a post hoc Newman-Keuls test. Vertical bars indicate the S.E.