Figure 6. Effects of CD4⁺CD25⁺ Treg cells deficiency on serum concentrations of the pro-inflammatory cytokines IL-6 (A), and TNF-á (B) (n = 10–12).

After the behavior tests, blood samples were collected from the mice by retro-orbital puncture. The serum prepared from each blood sample was then subjected to duplicate measurements for cytokine analysis using the mouse cytometric bead array (CBA). Data were analyzed using separate one-way ANOVA followed by a post hoc Newman-Keuls test. ** p<0.01, * p<0.05 vs. the stressed anti-CD25 Ab-treated group. Vertical bars indicate the S.E.