Figure 8. Effects of CD4⁺CD25⁺ Treg cells deficiency on serum concentrations of the Th2 cytokine IL-4 (A) and the Th17 cytokine IL-17A (B) (n = 10–12).

After the behavior tests, blood samples were collected from the mice by retro-orbital puncture. The serum prepared from each blood sample was subjected to duplicate measurements for cytokine analysis using the mouse cytometric bead array (CBA). Data were analyzed using separate one-way ANOVA followed by a post hoc Newman-Keuls test. *** p<0.00, ** p<0.01, * p<0.05 vs. the stressed anti-CD25 Ab-treated group. Vertical bars indicate the S.E.