Table 1. Primers used for PCR and sequencing of drug resistance-associated genes from K. pneumoniae isolates.

Target genes	Primer sequence (5′ to 3′)		Ampliconsize (bp)	Source ofreference
	Forward	Reverse
bla CTX-M-1	GGT TAA AAA ATC ACT GCG TC	TTA CAA ACC GTC GGT GAC GA	876	[2]
bla CTX-M-2	ATG ATG ACT CAG AGC ATT CG	TTA TTG CAT CAG AAA CCG TG	876	[29]
bla CTX-M-3	GTT GTT GTT ATT TCG TAT CTT CC	CGA TAA ACA AAA ACG GAA TG	934	[10]
bla CTX-M-8	ATG ATG AGA CAT CGC GTT AAG	CGG TGA CGA TTT TCG CGG CAG	864	[61]
bla CTX-M-9	GTG ACA AAG AGA GTG CAA CGG	ATG ATT CTC GCC GCT GAA GCC	850	[31]
bla CTX-M-10	GCA GCA CCA GTA AAG TGA TGG	GCG ATA TCG TTG GTG GTA CC	873	[30]
bla CTX-M-14	ACA ATG ACG CTG GCA GAA CTG	TTA CAG CCC TTC GGC GAT GA	512	[64]
bla CTX-M-25	CAC ACG AAT TGA ATG TTC AG	TCA CTC CAC ATG GTG AGT	924	[61]
bla SHV-group	TTT ATC GGC CYT CAC TCA AGG	GCT GCG GGC CGG ATA ACG	896	[32]
bla TEM	KAC AAT AAC CCT GRT AAA TGC	AGT ATA TAT GAG TAA ACT TGG	899	[32]
bla KPC	ATG TCA CTG TAT CGC CGT CT	TTT TCA GAG CCT TAC TGC CC	882	[8]
bla NDM	GGT TTG GCG ATC TGG TTT TC	CGG AAT GGC TCA TCA CGA TC	621	[51]
bla IMP	GGA ATA GAG TGG CTT AAT TCT C	CCA AAC CAC TAC GTT ATC	624	[27]
bla VIM	GGT CTC ATT GTC CGT GAT GGT GAT GAG	CTC GAT GAG AGT CCT TCT AGA G	271	[27]
bla OXA-48	TTG GTG GCA TCG ATT ATC GG	GAG CAC TTC TTT TGT GAT GGC	743	[47]
bla CMY	TGG CCA GAA CTG ACA GGC AAA	TTT CTC CTG AAC GTG GCT GGC	462	[65]
bla DHA	AAC TTT CAC AGG TGT GCT GGG T	CCG TAC GCA TAC TGG CTT TGC	405	[65]
bla FOX	AAC ATG GGG TAT CAG GGA GAT G	CAA AGC GCG TAA CCG GAT TGG	190	[65]
dhfr	GCC AAT CGG GTT ATT GGC AA	TGG GAA GAA GGC GTC ACC CTC	357	[15]
qnrA	ATT TCT CAC GCC AGG ATT TG	GAT CGG CAA AGG TTA GGT CA	627	[62]
qnrB	GAT CGT GAA AGC CAG AAA GG	ACG ATG CCT GGT AGT TGT CC	469	[62]
qnrC	GGG TTG TAC ATT TAT TGA ATC G	CAC CTA CCC ATT TAT TTT CA	307	[34]
qnrD	CGA GAT CAA TTTA CGG GGA ATA	AAC AAG CTG AAG CGC CTG	533	[33]
qnrS	ACG ACA TTC GTC AAC TGC AA	TAA ATT GGC ACC CTG TAG GC	417	[28]
aac(6′)-Ib-cr	TTG CGA TGC TCT ATG AGT GGC TA	CTC GAA TGC CTG GCG TGT TT	482	[35]
qepA	AAC TGC TTG AGC CCG TAG AT	GTC TAC GCC ATG GAC CTC AC	596	[34]
gyrA	CGA CCT TGC GAG AGA AAT	GTT CCA TCA GCC CTT CAA	626	[54]
parC	TAC GTC ATC ATG GAC AGG	GCC ACT TCA CGC AGG TTG	460	[37]
aacA4	ATG ACT GAG CAT GAC CTT GCG	TTA GGC ATC ACT GCG TGT TCG	540	[38]
aacC1	ATG GGC ATC ATT CGC ACA TGT AGG	TTA GGT GGC GGT ACT TGG GTC	873	[38]
aacC2	ATG CAT ACG CGG AAG GCA ATA AC	CTA ACC GGA AGG CTC GCA AG	861	[38]
aadA1	ATG AGG GAA GCG GTG ATC G	TTA TTT GCC GAC TAC CTT GGT G	792	[38]
aadB	ATG GAC ACA ACG CAG GTC GC	TTA GGC CGC ATA TCG CGA CC	534	[38]
aphA6	ATG GAA TTG CCC AAT ATT ATT C	TCA ATT CAA TTC ATC AAG TTT TA	781	[38]
armA	AGG TTG TTT CCA TTT CTG AG	TCT CTT CAT TCC CTT CTC C	591	[57]
rmtB	CCC AAA CAG ACC GTA GAG GC	CTC AAA CTC GGC GGG CAA GC	585	[57]
Integron I	GGC ATC CAA GCA CAA G	AAG CAG ACT TGA CCT GA	Variable	[63]