Table 2. Erythrophagocytosis Assay.
RBCN | RBCVL | |
a Group | bPositive macrophages (%) uptaking diseased erythrocytes | |
RBC | 2±1 | 3±1 |
Sensitized RBC | 3±2 | 52±5 |
RBCVL and RBCN (2×106), without or with sensitization by anti-O-AcSGP IgGVL and anti-O-AcSGP IgGNHS antibodies and processed for erythrophagocytosis assay as described in Materials and Methods. Results represent the mean ± S.D. of five separate determinations.
Positive macrophages (%) are the percentage of macrophages that ingested one or more erythrocytes and was used as the index of phagocytosis. Results represent the mean ± S.D. of five separate determinations.