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Polymer immunoblotting and immunocytochemistry of HaCaT keratinocytes grown for 14 days under control (+) or restricted (−) niacin (Nam) and exposed to photodamage. Cells treated with singlet oxygen stress (1O2) or solar simulated light (SSL) were analyzed by (A) Western blots using a PAR antibody and (B) immunostaining using a PAR antibody (green) and nuclear counterstaining with DAPI (blue).
