Table 1. SIRT gene expression in human skin-derived cultured cells.

	NHEK	HaCaT		A431		CF3
	dCt	dCt	Fold differenceto NHEK	dCt	Fold differenceto NHEK	dCt	Fold differenceto NHEK
	(Mean ± SEM)	(Mean ± SEM)	(Mean ± SEM)	(Mean ± SEM)	(Mean ± SEM)	(Mean ± SE004D)	(Mean ± SEM)
SIRT1	10.2±0.3	9.5±0.2	1.4±0.2	8.7±0.3	2.4±0.4*	9.6±0.3	1.5±0.1
SIRT2	8.9±0.2	10.0±0.3	0.5±0.1*	9.2±0.4	0.9±0.2	8.9±0.2	1.5±0.1
SIRT3	10.4±0.3	9.8±0.4	1.9±0.7	8.4±0.4	3.9±1.1*	9.7±0.4	1.7±0.3
SIRT4	16.0±0.3	16.6±0.4	0.7±0.1	16.3±0.3	0.6±0.1	15.2±0.5	2.0±0.2*
SIRT5	10.7±0.4	10.6±0.3	1.0±0.2	10.4±0.3	1.0±0.2	11.5±0.4	0.5±0.1*
SIRT6	9.4±0.2	9.5±0.4	0.8±0.1	10.2±0.4	0.7±0.1	10.0±0.3	0.7±0.1
SIRT7	9.1±0.3	9.3±0.4	0.6±0.1	8.6±0.4	1.6±0.3	10.7±0.5	0.4±0.1*

qPCR analyses of SIRT genes in cultures cells.

dCt = Ct differential to GAPDH. Difference from NHEK at *p<0.05 and a fold change of >1.5 or <0.67. n = 3 independent experiments analyzed in triplicate.