Table 1. SIRT gene expression in human skin-derived cultured cells.
NHEK | HaCaT | A431 | CF3 | ||||
dCt | dCt | Fold differenceto NHEK | dCt | Fold differenceto NHEK | dCt | Fold differenceto NHEK | |
(Mean ± SEM) | (Mean ± SEM) | (Mean ± SEM) | (Mean ± SEM) | (Mean ± SEM) | (Mean ± SE004D) | (Mean ± SEM) | |
SIRT1 | 10.2±0.3 | 9.5±0.2 | 1.4±0.2 | 8.7±0.3 | 2.4±0.4* | 9.6±0.3 | 1.5±0.1 |
SIRT2 | 8.9±0.2 | 10.0±0.3 | 0.5±0.1* | 9.2±0.4 | 0.9±0.2 | 8.9±0.2 | 1.5±0.1 |
SIRT3 | 10.4±0.3 | 9.8±0.4 | 1.9±0.7 | 8.4±0.4 | 3.9±1.1* | 9.7±0.4 | 1.7±0.3 |
SIRT4 | 16.0±0.3 | 16.6±0.4 | 0.7±0.1 | 16.3±0.3 | 0.6±0.1 | 15.2±0.5 | 2.0±0.2* |
SIRT5 | 10.7±0.4 | 10.6±0.3 | 1.0±0.2 | 10.4±0.3 | 1.0±0.2 | 11.5±0.4 | 0.5±0.1* |
SIRT6 | 9.4±0.2 | 9.5±0.4 | 0.8±0.1 | 10.2±0.4 | 0.7±0.1 | 10.0±0.3 | 0.7±0.1 |
SIRT7 | 9.1±0.3 | 9.3±0.4 | 0.6±0.1 | 8.6±0.4 | 1.6±0.3 | 10.7±0.5 | 0.4±0.1* |
qPCR analyses of SIRT genes in cultures cells.
dCt = Ct differential to GAPDH. Difference from NHEK at *p<0.05 and a fold change of >1.5 or <0.67. n = 3 independent experiments analyzed in triplicate.