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. 2012 Jul 31;7(7):e41928. doi: 10.1371/journal.pone.0041928

Figure 4. Flow-cytometry analysis of pertussis-specific CD3+CD4+ T-cells.

Figure 4

PBMCs of children 4 and 9 years of age pre-booster were stimulated with PT and Prn for 5 days and analyzed by 8-colour FACS analysis. T-cells which have proliferated upon stimulation (CFSE-) were characterized phenotypically by CD45RA and CCR7 and the effector memory cells (CD45RA- and CCR7-) were further analyzed functionally (IFN-γ+ and TNF-α+). The results of a representative child of 4 years and 9 years of age specific for PT (A) and Prn (B) are presented.