Table 2. Description of the putative reference gene (RGs), their TC numbers and abundances.

RGs	Putative Function ofGene Product	TC Release10.0	Number ofSequences	UniGene	Spike	Pericarp	Pistil	Seed	Average TPM	CV
RG1	S-adenosylmethioninedecarboxylase	TC158262	976	Hv.22842	2269	1411	863	1615	1540	0.377
RG2	elongation factor 1-alpha	TC176822	119	Hv.26096	421	245	39	429	284	0.648
RG3	Glyceraldehyde 3-phosphatedehydrogenase	TC161681	887	Hv.22848	1945	2578	1452	2575	2138	0.255
RG4	Glycine rich protein,RNA binding protein	TC163369	367	Hv.21398	1102	368	1295	1276	1010	0.432
RG5	Heat shock 70 KD protein(HSP70)	TC159018	837	Hv.19033	2107	1780	1216	1750	1713	0.215
RG6	ADP-ribosylation factor	TC177402	390	Hv.22835	1231	982	2041	835	1272	0.423
RG7	fructose-bisphosphatealdolase	BY839322	508	Hv.22920	1264	1780	1491	1457	1498	0.142
RG8	cytosolic heat shockprotein 90	TC180189	651	Hv.22798	1945	2148	1844	1276	1803	0.207
RG9	ubiquitin gene (mub1)	TC158625	366	Hv.22903	1556	1903	2473	1129	1765	0.322
RG10	acyl carrier protein III	TC170191	127	Hv.65	421	368	824	417	508	0.418

For in silico expression, profiles of each RG are available on the UniGene EST ProfileViewer across selected tissues related to barley caryopsis. Average TPM  =  average through all four tissues, CV  =  coefficient of variation of transcript occurrence for the four tissues.