Table 3. Background variation during the experimental process as assessed by calculating the standard deviation of repetitions across RGs and developmental stages.
SD | 5 DPA | 10 DPA | 15 DPA | 20 DPA | 25 DPA |
plant material | 2.606 | 1.967 | 1.363 | 1.349 | 1.243 |
parallel RNA extraction | 0.337 | 0.448 | 0.383 | 0.206 | 0.313 |
qPCR | 0.238 | 0.234 | 0.234 | 0.262 | 0.240 |
triplicate | 0.127 | 0.113 | 0.077 | 0.078 | 0.104 |
SD: standard deviation, DPA: number of days after anthesis when the caryopsis was harvested.