Table 1. Composition of the 5 sheep whole blood pools used for preparation of labile blood products.

	Collection date	Donor		PrPSc
		ID	Incubationperiod	CNS	LRS
Pool 1	161 dpi	D1	228 dpi	+	+
		D2	234 dpi	+	+
		D3	249 dpi	+	+
Pool 2	163 dpi	D4	225 dpi	+	+
		D5	217 dpi	+	+
		D6	221 dpi	+	+
Pool 3	168 dpi	D7	209 dpi	+	+
		D8	228 dpi	+	+
		D9	248 dpi	+	+
Pool 4	169 dpi	D10	209 dpi	+	+
		D11	256 dpi	+	+
		D12	226 dpi	+	+
Pool 5	170 dpi	D13	222 dpi	+	+
		D14	250 dpi	+	+
		D15	244 dpi	+	+

Each pool was obtained by mixing 600–700 mL of whole blood collected in 3 VRQ/VRQ sheep orally inoculated with PG127 classical scrapie isolate. After blood collection (collection date) each donor was monitored till the occurrence of clinical signs. Donors were culled when displaying locomotor difficulties that could impair their abilities to feed (incubation period). TSE was confirmed by detection of abnormal PrP in lymphoid tissues (LRS) and central nervous system (CNS) using immunohistochemistry and Western Blot.