Table 3. Classification and species richness of the fecal bacteria of healthy horses and horses affected by colitis.
Horse | Firmicutes | Bacteroidetes | Proteobacteria | Actinobacteria | Spirocaetes | Unclassified | Fusobacteria | Total | Species richness |
Healthy 1 | 39.5 | 12.2 | 42.7 | 4.2 | – | – | – | 6,602 | 189 |
Healthy 2 | 48.7 | 9.0 | 36.5 | 4.0 | 1.0 | – | – | 4,865 | 160 |
Healthy 3 | 78.0 | 8.9 | 3.4 | 7.6 | – | – | – | 17,170 | 230 |
Healthy 4 | 73.4 | 21.3 | – | 1.9 | 2.1 | – | – | 10,097 | 178 |
Healthy 5 | 73.1 | 13.8 | – | 3.5 | 8.7 | – | – | 3,438 | 142 |
Healthy 6 | 72.6 | 20.1 | – | 2.6 | 1.7 | 1.2 | – | 9,834 | 225 |
Mean | 68.1 | 14.2 | 10.1 | 4.5 | 1.9 | 0.2 | 0.0 | 52,006 | |
Colitis 1 | 49.2 | 46.0 | 3.1 | – | – | – | – | 4,782 | 138 |
Colitis 2 | 65.4 | 25.8 | 7.1 | – | – | – | – | 2,939 | 70 |
Colitis 3 | 51.9 | 38.5 | 3.0 | – | – | – | 5.1 | 5,089 | 154 |
Colitis 4 | 37.5 | 44.2 | 6.1 | – | – | – | 10.6 | 3,579 | 170 |
Colitis 5 | 33.1 | 50.7 | 7.5 | – | 2.3 | – | 5.5 | 7,677 | 240 |
Colitis 6 | 11.9 | 38.3 | 48.5 | – | – | – | – | 28,136 | 166 |
Colitis 7 | 33.5 | 27.3 | 2.4 | – | – | – | 35.6 | 7,025 | 120 |
Colitis 8 | 38.8 | 48.0 | 5.0 | – | – | – | 5.8 | 10,424 | 241 |
Colitis 9 | 28.3 | 69.5 | – | – | – | – | – | 7,253 | 115 |
Colitis 10 | 39.6 | 5.7 | 52.5 | 1.8 | – | – | – | 6,893 | 147 |
Mean | 30.3 | 40.0 | 18.74 | 0.4 | 0.3 | 0.0 | 5.2 | 83,797 | |
Total | 135,803 |
Classification of the intestinal bacteria of healthy horses and horses affected by colitis (in percentages) at the phylum level, total number of bacterial reads and species richness after data cleaning (pyrosequencing noise and chimera removal) and filtering (e-value of 30, minimum identity of 97% and minimum alignment of 75bp on MG-RAST).