Figure 3. CCTη does not influence NF-κB subcellular distribution.

HeLa cells were exposed to CCTη or scrambled (sc) siRNA for three days and stimulated with TNF (10 ng/ml) for indicated times. A: IκBα, p65, p50, CCTη and GAPDH were detected in cytoplasmic extracts and B: p65, p50 and Sp1 were detected in nuclear extracts by western blotting. Immunoblots are representative of three independent experiments.