Figure 4. CCTη knockdown increases NF-κB DNA binding.

HeLa cells were exposed to CCTη or scrambled (sc) siRNA for three days and stimulated with TNF (10 ng/ml) for indicated times. A: Nuclear extracts were analyzed by EMSA using a κB consensus dsDNA oligonucleotide. Data shown represent mean intensity ± SEM (n = 3). B: Supershift analysis of NF-κB complexes composed of p65/p50 hetero-dimers representative of two independent experiments. Ab, antibody; *, free probe.