Figure 1.

Genome-wide determination of mRNA decay rates in HFF cells and the iPS cells derived from them. (A) Exponential decay curves for HIST1H4B mRNA in HFF and iPS cells. Each symbol represents HIST1H4B mRNA abundance in a single replicate with closed symbols denoting mRNA abundance determined in HFF cell samples and open symbols denoting mRNA abundance in iPS cells. Three replicates were performed in each cell type at each time point. The P-value for each cell type indicates how well the data fits to the exponential decay curve (solid line). The dashed lines indicate the 95% confidence intervals (CI) for each curve. (B) Venn diagram showing the number of mRNA half-lives generated in each cell type. (C) Graph showing the distribution of 5481 mRNA half-lives in each cell type. (D) Graph showing median, 10^th, and 90^th percentiles for 5481 mRNA half-lives determined in HFF cells. (E) Graph showing median, 10^th, and 90^th percentile for 5481 mRNA half-lives determined in iPS cells. (F) Graph showing the fold difference in half-life between HFF and iPS cells for 5481 mRNAs.