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. 2012 Apr 4;18(1):825–833. doi: 10.2119/molmed.2012.00005

Figure 3.

Figure 3

Binding of histone H3 to macrophages is diminished by the opsonins Gas6 and MFG-E8. Macrophages were incubated with BSA (control), Gas6 or MFG-E8 (5 μg/mL) for 1 h and then washed three times, followed by incubation with Chromeo 488–conjugated histone H3 (H3-488) or Chromeo 488–conjugated BSA (BSA-488) (5 μg/mL) for 1 h. The cells were then washed again with PBS to remove unbound proteins. The quantities of proteins bound to macrophages were determined by a fluorescent plate reader. *p < 0.05 and ***p < 0.001 compared with the control group.