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Effect of axitinib on the ATPase activity of ABCG2. The vanadate-sensitive ATPase activity of ABCG2 was determined at different concentrations of axitinib. The inset shows the effect of lower concentrations of axitinib on ABCG2 ATPase activity. ATP hydrolysis was monitored by measuring the amount of inorganic phosphate released using a colorimetric assay.
