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Antithrombin secretion and retention under stress conditions. HEK-EBNA cells were transfected with 200 ng of WT/WT plasmid or cotransfected with 100 ng of WT plasmid and 100 ng of Δ393 plasmid (WT/London) and then treated at different pHs (A) and temperatures (B). Culture medium from cells were collected after treatment and analyzed by SDS-PAGE under reducing conditions by western blot with an antibody to antithrombin. Intracellular antithrombin was detected by immunofluorescence.
