Table 2.

Primers for the B cell receptor heavy chain immunoscope analysis.

Primer	Sequence
IgVH family
VH1	5′-TGGAGCTGAGSAGSCTGAGATCYGA-3′
VH2	5′-AACCCACASAGACCCTCAC-3′
VH3	5′-TCCCTKARACTCTCCTGTRCAGC-3′
VH4	5′-CTACAACCCSTCCCTCAAGAGT-3′
VH5	5′-CAGCACCGCCTACCTGCAGTGGAGC-3′
VH6	5′-TCCGGGGACAGTGTCTCT-3′
VH7	5′-CAGCACRGCATAYCTGCAGATCAG-3′
IgH γ chain	5′-6Fam-AAGTAGTCCTTGACCAGGCAGC-3′
IgH μ chain	5′-6Fam-GGAGACGAGGGGGAAAAGG-3′

The primers used to perform PCR in the immunoscope analysis of the 7 VH immunoglobulin families (VH1–VH7) for the IgG and IgM isotypes are shown.