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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mech Dev. 2012 May 15;129(5-8):136–146. doi: 10.1016/j.mod.2012.05.002

Fig.2. Spatiotemporal expression of Xmab21l3.

Fig.2

(A) Xmab21l3 expression is preceded by the onset of Xema expression. RT-PCR analysis of embryos collected at the indicated stages. The Spemann organizer marker chordin is first detected at stage 9 and increases dramatically at the initiation of gastrulation at stage 10. (B) RT-PCR analysis of embryos collected at the indicated stages. (C) RT-PCR of Xmab21l3 in explants of gastrula stage embryos. Xmab21l3 is expressed predominantly in the animal cap (AC) explants and to a lesser extent in ventral marginal zone (VMZ) explants of stage 10 embryos. DMZ, dorsal marginal zone. (D, D’) Xmab21l3 is expressed in the animal pole ectoderm of gastrula stage embryos. Animal and lateral views of whole mount in situ hybridization analysis of gastrula stage embryos. (E–E’’) Xmab21l3 is excluded from the dorsal neural tube (arrowheads). (E) Dorsal view of stage 20 embryos with anterior to the left. (E’) Posterior view of stage 20 embryos with anterior to the top. (E’’) Transverse cross-section of the dorsal neural tube. Dorsal is to the top.