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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mech Dev. 2012 May 15;129(5-8):136–146. doi: 10.1016/j.mod.2012.05.002

Fig.3. Xmab21l3 misexpression promotes dorsalization.

Fig.3

(A) Overexpression of Xmab21l3 in embryos causes head defects and ectopic tail-like lateral protrusions. Lateral (top) and dorsal (bottom) views of stage 35 embryos injected with 1ng of Xmab21l3 RNA in the animal pole of both blastomeres at the 2-cell stage. (B) Xmab21l3-induced lateral protrusions are positive for a somite-specific antigen. Whole mount immunohistochemistry of Xmab21l3 RNA-injected tadpoles with the 12/101 antibody (Kintner and Brockes, 1984). Arrows in (A) indicate protrusions; arrowheads in (B) indicate secondary axes. (C) Xmab21l3 overexpression promotes induction of dorsal markers chordin and goosecoid by Activin. RT-PCR analysis of uninjected and Xmab21l3 RNA-injected animal cap explants cultured until stage 10.5 in the absence or presence of a low dose of Activin (5ng/ml). (D) Xmab21l3 overexpression induces neural markers sox2 and sox3 in competent ectoderm. RTPCR analysis of animal cap explants from Xmab21l3 RNA-injected embryos cultured until stage 18. Xmab21l3 (l) and Xmab21l3 (h) indicate 0.5ng/embryo and 1ng/embryo doses, respectively, of injected Xmab21l3 RNA.