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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mech Dev. 2012 May 15;129(5-8):136–146. doi: 10.1016/j.mod.2012.05.002

Fig.4. Xmab21l3 does not directly repress transcription of a heterologous promoter.

Fig.4

(A) Gal4 constructs used in heterologous promoter repression assays. The constructs were designed for expression of the Gal4 DNA binding domain (1–147aa) alone or fused to full length Xmab21l3 (1–368) (Gal4-Xmab21l3), the Engrailed repressor domain (169–1057aa) (Gal4-En) or the VP16 activation domain (410–490aa) (Gal4-VP16). (B) Expression of a Gal4-Xmab21l3 fusion protein has little or no effect on Luciferase reporter gene expression. Gal4-En blocks reporter expression completely and Gal4-VP16 strongly upregulates reporter expression. Graph measures expression in RLU (Relative luciferase units).