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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mech Dev. 2012 May 15;129(5-8):136–146. doi: 10.1016/j.mod.2012.05.002

Fig.7. Analysis of effects of Xmab21l3 on BMP and FGF/ERK signaling.

Fig.7

(A) Injection of BMP2 RNA inhibits neuralization by Xmab21l3. Graphs show relative expression, assayed by RT-PCR, of the neural markers sox2 and sox3 in animal caps normalized to expression in uninjected explants. (B) Xmab21l3 misexpression inhibits expression of the BMP-responsive gene sizzled (szl), and induces expression of the dorsal marker fgf4. (C) Xmab21l3 misexpression inhibits expression of a Vent2-Luciferase reporter fusion protein; this construct includes a mutation in a TCF binding site that renders it insensitive to Wnt activation (Hikasa et al., 2010). (D) Xmab21l3 activates ERK1/2 signaling in animal cap explants. Western blot analysis using an antibody against di-phosphorylated ERK1/2 demonstrates that ERK1/2 is activated in response to treatment with FGF or following injection of Xmab21l3 RNA. The FGFR1 inhibitor SU5402 blocks ERK activation by FGF, Xmab21l3 and Xmab21l3SMT.