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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Fertil Steril. 2012 Jun 15;98(2):487–495.e1. doi: 10.1016/j.fertnstert.2012.05.015

Figure 2.

Figure 2

Florescence polarization assay for PDE3A activity. Increasing concentrations of the PDE9 inhibitor, BAY 73-6691, were evaluated for inhibitory properties against PDE3A activity at 0 µM (no inhibitor), 1 µM, 10 µM, 100 µM, and 1 mM (Red). Similarly, the cGMP analog, 8-Br-cGMP, was assayed at 0 µM (no inhibitor), 1 µM, 10 µM, 100 µM, 1 mM and 10 mM (Blue). No significant changes were observed for the PDE9 inhibitor. Different letters indicate a significant decrease in PDE3A and Z’ = 0.75 for both assays. Significant changes in activity were analyzed by ANOVA followed by posthoc comparison with Student-Newman-Keuls test with P<0.001. Error bars indicate the first standard deviation.