A. The first 4 Mb of LG IV (top) and Cen-IV (bottom) show colocalization of CenH3 and H3K9me3 at the centromere. Together with cytosine DNA methylation (5meC), H3K9me3 is also found in relatively short pericentric regions directly adjacent to the regions enriched for CenH3, and in dispersed regions of heterochromatin (e.g. around 1.1 Mb). H3K4me2 and –me3 are localized in gene-rich regions and excluded from the centromere. B. Binning CenH3 enrichment (reads/kb) reveals periodicity that is essentially absent from H3K9me3 enrichment. C. Phasing observed in WT strains (CenH3, A–C) is altered in dim-5 strains that lack H3K9me3 (CenH3, dim-5) but similar to WT in hpo strains (CenH3, hpo).