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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 May 8;12(8):1997–2007. doi: 10.1111/j.1600-6143.2012.04081.x

Figure 4. CsA-induced EMT and Nox2 protein expression were TGF-β1 dependent.

Figure 4

To determine the role of TGF-β1 in CNI-induced EMT and Nox2 expression, we treated wild type and TGF-β1-knockout tubular epithelial cells with CsA (10 μM) and evaluated cell morphology and Nox2 protein levels. CsA treatment was associated with EMT in wild type, but not in knockout cells (Panels b and d). Similarly, CsA did not increase Nox2 protein levels in knockout cells (Panels e and f), indicating that TGF-β1 is required for EMT and Nox2 synthesis in these experimental settings.