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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 May 8;12(8):1997–2007. doi: 10.1111/j.1600-6143.2012.04081.x

Figure 5. Fibrosis and Nox2 in the Rat Model of CsA Nephrotoxicity.

Figure 5

Adult male Fisher344 rats were randomized into five groups (n=6–8 in each group): Olive oil, Cyclosporine (15mg/kg/day), Cyclosporine + DPI (15mg/kg/day CsA + 0.5mg/kg/day), Cyclosporine + DPI (15mg/kg/day CsA + 1.0mg/kg/day) or Cyclosporine + Apocynin (15mg/kg/day + 16mg/kg/day). They received treatment for 30 days. Trichrome (a,b) studies demonstrated the typical striped fibrosis and cytoplasmic vacuolization that characterize chronic CNI toxicity. Immunoperoxidase studies showed that CsA nephrotoxicity was associated with upregulation of Nox2 (brown) in tubular and interstitial cells, and interstitial upregulation of α-SMA (purple) (c, d).