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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 May 8;12(8):1997–2007. doi: 10.1111/j.1600-6143.2012.04081.x

Figure 6. Inhibition of Nox2 was associated with reduced CsA-induced Fibrogenesis.

Figure 6

Immunoblot analyses examining the expression of Nox2, fibrosis (α-SMA), oxidative stress (nitrotyrosine), and the profibrotic signaling molecule, phosphorylated Smad3 and total Smad3) in rats treated with high dose CsA (15 mg/kg/24h for 30 days), CsA + Apocynin (16 mg/kg/24h) and CsA + DPI (0.5 or 1 mg/kg/24h). Panel a shows representative blots while Panel b represents normalized ratios of proteins to GAPDH. The studies showed that CsA increased fibrogenesis (Nox2, α-SMA, nitrotyrosine, P-smad3 and T-smad3) compared to no treatment. Nox2 inhibitors were associated with reduced fibrogenesis, which was more pronounced with DPI than apocynin.