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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arthritis Rheum. 2012 Aug;64(8):2471–2481. doi: 10.1002/art.34452

Figure 6. CCL21 enhances blood vessel growth in matrigel plugs in vivo.

Figure 6

A. CCL21 (4 μg) induced angiogenesis in the matrigel plugs to a significantly greater degree compared to PBS control. Matrigel containing bFGF (100 ng) served as positive control. The values represent the concentration of hemoglobin (g/dl) / plug weight (g) ± SE, with n=10. B. The histology was quantified on a score of 0-4 scale in matrigel plugs that contained PBS, CCL21 or bFGF employing H&E and Masson’s trichrome staining. A representative assay shows H&E (C, F and I) and Masson’s trichrome (D, G and J) staining of blood vessels in paraffin sections of matrigel plugs containing PBS (C and D), CCL21 (F and G) and bFGF (I and J) that was histologically scored in B (original magnification × 200). Matrigel plugs containing PBS (E), CCL21 (H) or bFGF (K) were stained with VEGFR2 to distinguish EPCs recruitment in this study. Values demonstrate mean ± SE, with n=5. * represents p<0.05.