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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 May 11;12(8):2017–2028. doi: 10.1111/j.1600-6143.2012.04073.x

Figure 2. Intense cell infiltration in B6.Kd cardiac allografts retrieved from B6.CCR5−/− recipients.

Figure 2

B6.Kd cardiac allografts were excised from wild-type C57BL/6, B6.CCR5−/− and CD8−/−/CCR5−/− recipients on day 10 post-transplant (5 individual grafts per group), were digested and stained with leukocyte-specific antibodies. Flow cytometry analyses of anti-CD45 and anti-CD4, anti-CD8, anti-F4/80 and anti-Ly-6G mAb stained cells from digested grafts were performed to determine numbers of CD4 T cells, CD8 T cells, macrophages and neutrophils infiltrating the allografts. Data indicate mean number of cells/mg graft tissue ± SEM and are representative of two individual experiments. (**p<0.01; n.s.= p>0.05).